Part:BBa_K2045000
FhuA-GBP:Ferrichrome Iron outer membrane transporter-gold binding peptide
In order to effectively bind gold nanoparticles to E.coli, Ferrichrome Iron outer membrane transporter was used as a binding intermediate between the E.coli cell and gold nanoparticles. This was done by improving iGEM BCCS-Bristol’s part, BBa_K259000, by fusing a gold binding peptide with their part which was FhuA.
We sought to synthesize and display the gold nanoparticles on the surface of E.coli cells. A gold binding peptide is a series of amino acids that have an affinity to gold, mostly sulfide based amino acids, since sulfide groups have a natural chemical affinity to gold. This property makes it a vital part for iGEM Concordia for effective attachment of gold nanoparticles onto the cell surface of E.coli. In order to accomplish this; gold nanoparticles were displayed by fusing the gold binding peptide with FhuA. FhuA is a ferrichrome Iron outer membrane transporter which we fused GBP with to create FhuA-GBP. The binding of gold nanoparticles occurred in cells expressing FhuA-GBP. These findings demonstrate the effectiveness of the proposed method in the synthesis and display of gold nanoparticles.This part has many future applications and will allow for the attachment of gold nanoparticles to many kinds of protein structures and will be a future key player in the emerging use of nanotechnology in synthetic biology.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 682
Illegal AgeI site found at 820
Illegal AgeI site found at 1225
Illegal AgeI site found at 1999 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 724
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